Review

ov90 (ATCC)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC ov90

( A ) Schematic illustration of the collagen invasion assay. ( B ) Representative images of spheroid collagen invasion. Scale bars, 200 μm. ( C ) Different angles of 3D collagen spheroid invasion. Red-stained mesothelial cells invaded the collagen first (red arrow), followed by green-stained <t>OV90</t> cells, which used the same route (white arrow). Scale bar, 50 μm. ( D ) Scheme showing spheroid invasion areas into the collagen layer and original spheroid area. ( E ) Bar plots of the spheroid invasion areas. The invasion area of ACMS was significantly larger compared with spheroids with only EOC cells (125 versus 480%, extended from the original spheroid area at 48 hours). The invasion area of EOC cells from ACMSs was significantly larger compared to those with only EOC spheroids (128% versus 223%, at 72 hours). Each dot represents each experiment. ( F ) Scheme of the mesothelial clearance assay. ( G ) Representative images of several different ACMS invasion timings into the mesothelial layer. (a) Initial phase, (b) attached phase, (c) invasion phase, and (d) moving phase. Scale bar, 200 μm. ( H ) The mesothelial clearance area was significantly larger for ACMSs than that of spheroids with only OV90. ( I ) Representative images of the invasion front from ACMSs into the mesothelial layer. Scale bar, 200 μm. ( J ) High-magnification images of the ACMS and mesothelial layer invasion front. The blue-stained mesothelial cells close to the EOC invasion area were of a spindle-like shape, but originally, they were cobblestone shaped. The morphology was similar to that of red-stained mesothelial cells that interacted with EOC cells in ACMSs. Scale bar, 100 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Ov90, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 483 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ov90/product/ATCC
Average 97 stars, based on 483 article reviews

ov90 - by Bioz Stars, 2026-03

97/100 stars

Images

1) Product Images from "Mesothelial cells promote peritoneal invasion and metastasis of ascites-derived ovarian cancer cells through spheroid formation"

Article Title: Mesothelial cells promote peritoneal invasion and metastasis of ascites-derived ovarian cancer cells through spheroid formation

Journal: Science Advances

doi: 10.1126/sciadv.adu5944

Figure Legend Snippet: ( A ) Schematic illustration of the collagen invasion assay. ( B ) Representative images of spheroid collagen invasion. Scale bars, 200 μm. ( C ) Different angles of 3D collagen spheroid invasion. Red-stained mesothelial cells invaded the collagen first (red arrow), followed by green-stained OV90 cells, which used the same route (white arrow). Scale bar, 50 μm. ( D ) Scheme showing spheroid invasion areas into the collagen layer and original spheroid area. ( E ) Bar plots of the spheroid invasion areas. The invasion area of ACMS was significantly larger compared with spheroids with only EOC cells (125 versus 480%, extended from the original spheroid area at 48 hours). The invasion area of EOC cells from ACMSs was significantly larger compared to those with only EOC spheroids (128% versus 223%, at 72 hours). Each dot represents each experiment. ( F ) Scheme of the mesothelial clearance assay. ( G ) Representative images of several different ACMS invasion timings into the mesothelial layer. (a) Initial phase, (b) attached phase, (c) invasion phase, and (d) moving phase. Scale bar, 200 μm. ( H ) The mesothelial clearance area was significantly larger for ACMSs than that of spheroids with only OV90. ( I ) Representative images of the invasion front from ACMSs into the mesothelial layer. Scale bar, 200 μm. ( J ) High-magnification images of the ACMS and mesothelial layer invasion front. The blue-stained mesothelial cells close to the EOC invasion area were of a spindle-like shape, but originally, they were cobblestone shaped. The morphology was similar to that of red-stained mesothelial cells that interacted with EOC cells in ACMSs. Scale bar, 100 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Techniques Used: Invasion Assay, Staining

( A ) Scheme for the ex vivo model using the resected omentum. ( B ) Images of metastases on the omentum in the ex vivo model. Red arrows denote the metastasis sites. Scale bars, 2 mm. ( C ) Bar graph showing the number of metastases in each group (OV90, n = 23; SKOV3, n = 26). ( D ) Representative confocal microscopy images of green-stained SKOV3 and red-stained mesothelial cells in the ex vivo model. Scale bars, 200 μm. ( E ) Bar graphs showing the invasion length from the original spheroid into omentum tissue. Each dot represents the number of spheroids. ( F ) Scheme of the malignant ascites in vivo model. ( G ) Spheroids composed of green-stained SKOV3 and red-stained mesothelial cells using a confocal microscopy in vivo model at 2 or 4 hours later after injection. Scale bars, 100 μm. ( H ) Differences in the formation of spheroids in ascites when mice were injected with OV90 with or without HPMCs. Scale bars, 100 μm. ( I ) Bar graph showing the number of spheroids detected in ascites. ( J ) Images of the omentum using fluorescence microscopy 1 or 2 weeks after green-stained OV90 injection. Scale bars, 1 mm. ( K ) Bar graph showing the number of omentum metastases with and without mesothelial cell injection. ( L ) Metastasis area of the omentum in different compositions of the spheroids. ( M ) Magnification images of the omentum metastasis sites. Scale bar, 750 μm. ( N ) Magnification of the mesenteric metastases. Scale bar, 2.5 mm. ( O ) Scheme for the assay of tumor burden over time in the in vivo model. ( P ) Representative bioluminescence images after luc-OV90 injection with or without mesothelial cells. ( Q ) Total tumor flux using bioluminescence images. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Figure Legend Snippet: ( A ) Scheme for the ex vivo model using the resected omentum. ( B ) Images of metastases on the omentum in the ex vivo model. Red arrows denote the metastasis sites. Scale bars, 2 mm. ( C ) Bar graph showing the number of metastases in each group (OV90, n = 23; SKOV3, n = 26). ( D ) Representative confocal microscopy images of green-stained SKOV3 and red-stained mesothelial cells in the ex vivo model. Scale bars, 200 μm. ( E ) Bar graphs showing the invasion length from the original spheroid into omentum tissue. Each dot represents the number of spheroids. ( F ) Scheme of the malignant ascites in vivo model. ( G ) Spheroids composed of green-stained SKOV3 and red-stained mesothelial cells using a confocal microscopy in vivo model at 2 or 4 hours later after injection. Scale bars, 100 μm. ( H ) Differences in the formation of spheroids in ascites when mice were injected with OV90 with or without HPMCs. Scale bars, 100 μm. ( I ) Bar graph showing the number of spheroids detected in ascites. ( J ) Images of the omentum using fluorescence microscopy 1 or 2 weeks after green-stained OV90 injection. Scale bars, 1 mm. ( K ) Bar graph showing the number of omentum metastases with and without mesothelial cell injection. ( L ) Metastasis area of the omentum in different compositions of the spheroids. ( M ) Magnification images of the omentum metastasis sites. Scale bar, 750 μm. ( N ) Magnification of the mesenteric metastases. Scale bar, 2.5 mm. ( O ) Scheme for the assay of tumor burden over time in the in vivo model. ( P ) Representative bioluminescence images after luc-OV90 injection with or without mesothelial cells. ( Q ) Total tumor flux using bioluminescence images. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Techniques Used: Ex Vivo, Confocal Microscopy, Staining, In Vivo, Injection, Fluorescence, Microscopy

( A ) Schematic showing the comparison between RNA expression in OV90 and mesothelial cells. ( B and C ) PCA plot of (B) OV90 and (C) HPMCs. ( D ) Volcano plot and clustering of RNA expression changes in OV90. The red line indicates an adjusted P value <0.05. ( E ) Volcano plot and clustering of RNA expression changes in HPMCs. The red line represents an adjusted P value <0.05. The right side of the volcano plot represents a fold change. ( F ) Significant up-regulated pathway changes in mesothelial cells after interaction with OV90 in KEGG. ( G ) Significant up-regulated pathway changes in mesothelial cells after interaction with OV90 in the GO term. ( H ) Significant down-regulated pathway changes in mesothelial cells after interaction with OV90 in KEGG. ( I ) Significant down-regulated pathway changes in mesothelial cells after interaction with OV90 in the GO term. ( J and K ) PROGENy pathway activity analysis of the ascites samples of the Zheng et al. EOC scRNA-seq dataset revealed high TGF-β pathway activity in both EOC and mesothelial cells. ( L ) Bar plot showing the concentration of TGF-β1 in the supernatant from HPMCs, TGF-β1–stimulated HPMCs, and OV90 cells. ( M ) Scheme of an invadopodium in a mesothelial cell. ( N ) Immunofluorescence images of a single cell invading the collagen layer using invadopodium formation. Green, cortactin; red, phalloidin. Scale bars, 10 μm. ( O ) The number of invadopodia was significantly higher in TGF-β1–stimulated mesothelial cells. ( P ) Strategy to detect candidates with a high invasion ability in mesothelial cells. ( Q ) Western blot analysis of fascin-1 and several proteins related to invadopodium formation. ( R ) Immunofluorescence images of fascin-1 or myosin X (green) in TGF-β1–stimulated mesothelial cells. Scale bars, 5 μm. FACS, fluorescence-activated cell sorting; FC, fold change. *** P < 0.001.

Figure Legend Snippet: ( A ) Schematic showing the comparison between RNA expression in OV90 and mesothelial cells. ( B and C ) PCA plot of (B) OV90 and (C) HPMCs. ( D ) Volcano plot and clustering of RNA expression changes in OV90. The red line indicates an adjusted P value <0.05. ( E ) Volcano plot and clustering of RNA expression changes in HPMCs. The red line represents an adjusted P value <0.05. The right side of the volcano plot represents a fold change. ( F ) Significant up-regulated pathway changes in mesothelial cells after interaction with OV90 in KEGG. ( G ) Significant up-regulated pathway changes in mesothelial cells after interaction with OV90 in the GO term. ( H ) Significant down-regulated pathway changes in mesothelial cells after interaction with OV90 in KEGG. ( I ) Significant down-regulated pathway changes in mesothelial cells after interaction with OV90 in the GO term. ( J and K ) PROGENy pathway activity analysis of the ascites samples of the Zheng et al. EOC scRNA-seq dataset revealed high TGF-β pathway activity in both EOC and mesothelial cells. ( L ) Bar plot showing the concentration of TGF-β1 in the supernatant from HPMCs, TGF-β1–stimulated HPMCs, and OV90 cells. ( M ) Scheme of an invadopodium in a mesothelial cell. ( N ) Immunofluorescence images of a single cell invading the collagen layer using invadopodium formation. Green, cortactin; red, phalloidin. Scale bars, 10 μm. ( O ) The number of invadopodia was significantly higher in TGF-β1–stimulated mesothelial cells. ( P ) Strategy to detect candidates with a high invasion ability in mesothelial cells. ( Q ) Western blot analysis of fascin-1 and several proteins related to invadopodium formation. ( R ) Immunofluorescence images of fascin-1 or myosin X (green) in TGF-β1–stimulated mesothelial cells. Scale bars, 5 μm. FACS, fluorescence-activated cell sorting; FC, fold change. *** P < 0.001.

Techniques Used: Comparison, RNA Expression, Activity Assay, Concentration Assay, Immunofluorescence, Single Cell, Western Blot, Fluorescence, FACS

( A ) Representative images of collagen invasion of spheroids composed of HPMCs or TGF-β1–stimulated HPMCs. Scale bars, 200 μm. ( B ) The bar graph showing TGF-β1–stimulated HPMCs showed a higher invasion ability into the collagen layer. ( C ) Images of migration or invasion cells using the Transwell assay. Scale bar, 200 μm. ( D ) Linear plots showing that TGF-β1–stimulated HPMCs had a higher migration and invasion ability compared with the control HPMCs. ( E ) Bar graph showing the concentration of TGF-β1 in the supernatant in EOC cells when inhibiting TGF-β1 with siRNA. ( F ) Representative images of spheroid collagen invasion. Scale bars, 100 μm. ( G ) Bar graphs showing that inhibition of TGF-β1 by siRNA in OV90 cells or the TGF-β1 receptor blocker in HPMCs reduced the invasion ability of ACMSs compared with the control. ( H and I ) Differences in the spheroids in ascites when mice were injected with OV90 (green) and HPMCs (red). The number of spheroids was significantly decreased when OV90 cells were treated with siRNA for TGF-β1 than those with si-control. Scale bars, 100 μm. ( J and K ) Representative images of metastases on the omentum and bar graph showing the metastasis area on the omentum. Scale bars, 1000 μm. ( L ) Bar graph showing the TGF-β1 concentration in the culture supernatant in sh-control– or TGF-β1–transduced OV90 cells. ( M and N ) Representative images and bar graph showing the omental metastatic area ( n = 8). Scale bars, 1000 μm. ( O and P ) Representative images of confocal imaging of the omental micrometastasis area and bar graph showing the invasion depth of mesothelial cells from the metastatic border. Scale bars, 100 μm. ( Q and R ) Representative images and bar graph showing that both the number and size of spheroids in ascites were significantly decreased in mice injected with sh-TGF-β1 no. 1 or 2 O90 cells compared with sh-control mice. Scale bars, 100 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Figure Legend Snippet: ( A ) Representative images of collagen invasion of spheroids composed of HPMCs or TGF-β1–stimulated HPMCs. Scale bars, 200 μm. ( B ) The bar graph showing TGF-β1–stimulated HPMCs showed a higher invasion ability into the collagen layer. ( C ) Images of migration or invasion cells using the Transwell assay. Scale bar, 200 μm. ( D ) Linear plots showing that TGF-β1–stimulated HPMCs had a higher migration and invasion ability compared with the control HPMCs. ( E ) Bar graph showing the concentration of TGF-β1 in the supernatant in EOC cells when inhibiting TGF-β1 with siRNA. ( F ) Representative images of spheroid collagen invasion. Scale bars, 100 μm. ( G ) Bar graphs showing that inhibition of TGF-β1 by siRNA in OV90 cells or the TGF-β1 receptor blocker in HPMCs reduced the invasion ability of ACMSs compared with the control. ( H and I ) Differences in the spheroids in ascites when mice were injected with OV90 (green) and HPMCs (red). The number of spheroids was significantly decreased when OV90 cells were treated with siRNA for TGF-β1 than those with si-control. Scale bars, 100 μm. ( J and K ) Representative images of metastases on the omentum and bar graph showing the metastasis area on the omentum. Scale bars, 1000 μm. ( L ) Bar graph showing the TGF-β1 concentration in the culture supernatant in sh-control– or TGF-β1–transduced OV90 cells. ( M and N ) Representative images and bar graph showing the omental metastatic area ( n = 8). Scale bars, 1000 μm. ( O and P ) Representative images of confocal imaging of the omental micrometastasis area and bar graph showing the invasion depth of mesothelial cells from the metastatic border. Scale bars, 100 μm. ( Q and R ) Representative images and bar graph showing that both the number and size of spheroids in ascites were significantly decreased in mice injected with sh-TGF-β1 no. 1 or 2 O90 cells compared with sh-control mice. Scale bars, 100 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Techniques Used: Migration, Transwell Assay, Control, Concentration Assay, Inhibition, Injection, Imaging

( A and B ) Violin plots showing the expression of invadopodium-related genes across cell components in ascites on the basis of two different scRNA-seq datasets from Izar et al. and Zheng et al. . In the dataset of (A), mesothelial cells are classified as fibroblasts. ( C ) Collagen degradation assay. The thickness represents the cell invasion ability. Scale bars, 400 μm. ( D ) Bar graph showing the thickness of remnant collagen 48 hours after incubation. ( E ) Bar graph showing the number of invadopodia. sh-Fascin-1 or sh-myosin X inhibited invadopodium maturation. ( F and G ) 3D images and bar graph showing that spheroids invade collagen with shRNA-induced mesothelial cells (green) and OV90 (red). The invasion ability of mesothelial cells was significantly inhibited by sh- FSCN1 or sh- MYO10 . Scale bars, 200 μm. ( H ) Scheme of the malignant ascites in vivo model using shRNA-treated HPMCs. ( I and J ) Images and bar graph showing the differences in the metastasis area on the omentum from mice 1 week after the injection of OV90 with or without sh-induced mesothelial cells. Scale bars, 1 mm. ( K ) Representative IHC image of mouse tissue with fascin-1. Invasive stromal cells strongly expressed fascin-1. Scale bar, 100 μm. ( L ) IHC of metastasis samples in clinical samples. Fascin-1–positive stromal cells were present in the tumor-invasive regions. Scale bar, 100 μm. ( M ) Kaplan-Meier plot showing the patient’s progression-free survival depending on fascin-1 expression in stromal cells or cancer cells. Fascin-1 expression in stromal cells in metastasis samples was significantly related to a worse prognosis ( P = 0.030). * P < 0.05, ** P < 0.01, and *** P < 0.001.

Figure Legend Snippet: ( A and B ) Violin plots showing the expression of invadopodium-related genes across cell components in ascites on the basis of two different scRNA-seq datasets from Izar et al. and Zheng et al. . In the dataset of (A), mesothelial cells are classified as fibroblasts. ( C ) Collagen degradation assay. The thickness represents the cell invasion ability. Scale bars, 400 μm. ( D ) Bar graph showing the thickness of remnant collagen 48 hours after incubation. ( E ) Bar graph showing the number of invadopodia. sh-Fascin-1 or sh-myosin X inhibited invadopodium maturation. ( F and G ) 3D images and bar graph showing that spheroids invade collagen with shRNA-induced mesothelial cells (green) and OV90 (red). The invasion ability of mesothelial cells was significantly inhibited by sh- FSCN1 or sh- MYO10 . Scale bars, 200 μm. ( H ) Scheme of the malignant ascites in vivo model using shRNA-treated HPMCs. ( I and J ) Images and bar graph showing the differences in the metastasis area on the omentum from mice 1 week after the injection of OV90 with or without sh-induced mesothelial cells. Scale bars, 1 mm. ( K ) Representative IHC image of mouse tissue with fascin-1. Invasive stromal cells strongly expressed fascin-1. Scale bar, 100 μm. ( L ) IHC of metastasis samples in clinical samples. Fascin-1–positive stromal cells were present in the tumor-invasive regions. Scale bar, 100 μm. ( M ) Kaplan-Meier plot showing the patient’s progression-free survival depending on fascin-1 expression in stromal cells or cancer cells. Fascin-1 expression in stromal cells in metastasis samples was significantly related to a worse prognosis ( P = 0.030). * P < 0.05, ** P < 0.01, and *** P < 0.001.

Techniques Used: Expressing, Degradation Assay, Incubation, shRNA, In Vivo, Injection

Image Search Results

Journal: Science Advances

Article Title: Mesothelial cells promote peritoneal invasion and metastasis of ascites-derived ovarian cancer cells through spheroid formation

doi: 10.1126/sciadv.adu5944

Figure Lengend Snippet: ( A ) Schematic illustration of the collagen invasion assay. ( B ) Representative images of spheroid collagen invasion. Scale bars, 200 μm. ( C ) Different angles of 3D collagen spheroid invasion. Red-stained mesothelial cells invaded the collagen first (red arrow), followed by green-stained OV90 cells, which used the same route (white arrow). Scale bar, 50 μm. ( D ) Scheme showing spheroid invasion areas into the collagen layer and original spheroid area. ( E ) Bar plots of the spheroid invasion areas. The invasion area of ACMS was significantly larger compared with spheroids with only EOC cells (125 versus 480%, extended from the original spheroid area at 48 hours). The invasion area of EOC cells from ACMSs was significantly larger compared to those with only EOC spheroids (128% versus 223%, at 72 hours). Each dot represents each experiment. ( F ) Scheme of the mesothelial clearance assay. ( G ) Representative images of several different ACMS invasion timings into the mesothelial layer. (a) Initial phase, (b) attached phase, (c) invasion phase, and (d) moving phase. Scale bar, 200 μm. ( H ) The mesothelial clearance area was significantly larger for ACMSs than that of spheroids with only OV90. ( I ) Representative images of the invasion front from ACMSs into the mesothelial layer. Scale bar, 200 μm. ( J ) High-magnification images of the ACMS and mesothelial layer invasion front. The blue-stained mesothelial cells close to the EOC invasion area were of a spindle-like shape, but originally, they were cobblestone shaped. The morphology was similar to that of red-stained mesothelial cells that interacted with EOC cells in ACMSs. Scale bar, 100 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: The OV90 (RRID: CVCL_3768), SKOV3 (CVCL_0532), OVCAR3 (CVCL_0465), ES2 (CVCL_3509), and BJ (CRL_2522) cells were obtained from American Type Culture Collection (Manassas, VA).

Techniques: Invasion Assay, Staining

Journal: Science Advances

Article Title: Mesothelial cells promote peritoneal invasion and metastasis of ascites-derived ovarian cancer cells through spheroid formation

doi: 10.1126/sciadv.adu5944

Figure Lengend Snippet: ( A ) Scheme for the ex vivo model using the resected omentum. ( B ) Images of metastases on the omentum in the ex vivo model. Red arrows denote the metastasis sites. Scale bars, 2 mm. ( C ) Bar graph showing the number of metastases in each group (OV90, n = 23; SKOV3, n = 26). ( D ) Representative confocal microscopy images of green-stained SKOV3 and red-stained mesothelial cells in the ex vivo model. Scale bars, 200 μm. ( E ) Bar graphs showing the invasion length from the original spheroid into omentum tissue. Each dot represents the number of spheroids. ( F ) Scheme of the malignant ascites in vivo model. ( G ) Spheroids composed of green-stained SKOV3 and red-stained mesothelial cells using a confocal microscopy in vivo model at 2 or 4 hours later after injection. Scale bars, 100 μm. ( H ) Differences in the formation of spheroids in ascites when mice were injected with OV90 with or without HPMCs. Scale bars, 100 μm. ( I ) Bar graph showing the number of spheroids detected in ascites. ( J ) Images of the omentum using fluorescence microscopy 1 or 2 weeks after green-stained OV90 injection. Scale bars, 1 mm. ( K ) Bar graph showing the number of omentum metastases with and without mesothelial cell injection. ( L ) Metastasis area of the omentum in different compositions of the spheroids. ( M ) Magnification images of the omentum metastasis sites. Scale bar, 750 μm. ( N ) Magnification of the mesenteric metastases. Scale bar, 2.5 mm. ( O ) Scheme for the assay of tumor burden over time in the in vivo model. ( P ) Representative bioluminescence images after luc-OV90 injection with or without mesothelial cells. ( Q ) Total tumor flux using bioluminescence images. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: The OV90 (RRID: CVCL_3768), SKOV3 (CVCL_0532), OVCAR3 (CVCL_0465), ES2 (CVCL_3509), and BJ (CRL_2522) cells were obtained from American Type Culture Collection (Manassas, VA).

Techniques: Ex Vivo, Confocal Microscopy, Staining, In Vivo, Injection, Fluorescence, Microscopy

Journal: Science Advances

Article Title: Mesothelial cells promote peritoneal invasion and metastasis of ascites-derived ovarian cancer cells through spheroid formation

doi: 10.1126/sciadv.adu5944

Figure Lengend Snippet: ( A ) Schematic showing the comparison between RNA expression in OV90 and mesothelial cells. ( B and C ) PCA plot of (B) OV90 and (C) HPMCs. ( D ) Volcano plot and clustering of RNA expression changes in OV90. The red line indicates an adjusted P value <0.05. ( E ) Volcano plot and clustering of RNA expression changes in HPMCs. The red line represents an adjusted P value <0.05. The right side of the volcano plot represents a fold change. ( F ) Significant up-regulated pathway changes in mesothelial cells after interaction with OV90 in KEGG. ( G ) Significant up-regulated pathway changes in mesothelial cells after interaction with OV90 in the GO term. ( H ) Significant down-regulated pathway changes in mesothelial cells after interaction with OV90 in KEGG. ( I ) Significant down-regulated pathway changes in mesothelial cells after interaction with OV90 in the GO term. ( J and K ) PROGENy pathway activity analysis of the ascites samples of the Zheng et al. EOC scRNA-seq dataset revealed high TGF-β pathway activity in both EOC and mesothelial cells. ( L ) Bar plot showing the concentration of TGF-β1 in the supernatant from HPMCs, TGF-β1–stimulated HPMCs, and OV90 cells. ( M ) Scheme of an invadopodium in a mesothelial cell. ( N ) Immunofluorescence images of a single cell invading the collagen layer using invadopodium formation. Green, cortactin; red, phalloidin. Scale bars, 10 μm. ( O ) The number of invadopodia was significantly higher in TGF-β1–stimulated mesothelial cells. ( P ) Strategy to detect candidates with a high invasion ability in mesothelial cells. ( Q ) Western blot analysis of fascin-1 and several proteins related to invadopodium formation. ( R ) Immunofluorescence images of fascin-1 or myosin X (green) in TGF-β1–stimulated mesothelial cells. Scale bars, 5 μm. FACS, fluorescence-activated cell sorting; FC, fold change. *** P < 0.001.

Article Snippet: The OV90 (RRID: CVCL_3768), SKOV3 (CVCL_0532), OVCAR3 (CVCL_0465), ES2 (CVCL_3509), and BJ (CRL_2522) cells were obtained from American Type Culture Collection (Manassas, VA).

Techniques: Comparison, RNA Expression, Activity Assay, Concentration Assay, Immunofluorescence, Single Cell, Western Blot, Fluorescence, FACS

Journal: Science Advances

Article Title: Mesothelial cells promote peritoneal invasion and metastasis of ascites-derived ovarian cancer cells through spheroid formation

doi: 10.1126/sciadv.adu5944

Figure Lengend Snippet: ( A ) Representative images of collagen invasion of spheroids composed of HPMCs or TGF-β1–stimulated HPMCs. Scale bars, 200 μm. ( B ) The bar graph showing TGF-β1–stimulated HPMCs showed a higher invasion ability into the collagen layer. ( C ) Images of migration or invasion cells using the Transwell assay. Scale bar, 200 μm. ( D ) Linear plots showing that TGF-β1–stimulated HPMCs had a higher migration and invasion ability compared with the control HPMCs. ( E ) Bar graph showing the concentration of TGF-β1 in the supernatant in EOC cells when inhibiting TGF-β1 with siRNA. ( F ) Representative images of spheroid collagen invasion. Scale bars, 100 μm. ( G ) Bar graphs showing that inhibition of TGF-β1 by siRNA in OV90 cells or the TGF-β1 receptor blocker in HPMCs reduced the invasion ability of ACMSs compared with the control. ( H and I ) Differences in the spheroids in ascites when mice were injected with OV90 (green) and HPMCs (red). The number of spheroids was significantly decreased when OV90 cells were treated with siRNA for TGF-β1 than those with si-control. Scale bars, 100 μm. ( J and K ) Representative images of metastases on the omentum and bar graph showing the metastasis area on the omentum. Scale bars, 1000 μm. ( L ) Bar graph showing the TGF-β1 concentration in the culture supernatant in sh-control– or TGF-β1–transduced OV90 cells. ( M and N ) Representative images and bar graph showing the omental metastatic area ( n = 8). Scale bars, 1000 μm. ( O and P ) Representative images of confocal imaging of the omental micrometastasis area and bar graph showing the invasion depth of mesothelial cells from the metastatic border. Scale bars, 100 μm. ( Q and R ) Representative images and bar graph showing that both the number and size of spheroids in ascites were significantly decreased in mice injected with sh-TGF-β1 no. 1 or 2 O90 cells compared with sh-control mice. Scale bars, 100 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: The OV90 (RRID: CVCL_3768), SKOV3 (CVCL_0532), OVCAR3 (CVCL_0465), ES2 (CVCL_3509), and BJ (CRL_2522) cells were obtained from American Type Culture Collection (Manassas, VA).

Techniques: Migration, Transwell Assay, Control, Concentration Assay, Inhibition, Injection, Imaging

Journal: Science Advances

Article Title: Mesothelial cells promote peritoneal invasion and metastasis of ascites-derived ovarian cancer cells through spheroid formation

doi: 10.1126/sciadv.adu5944

Figure Lengend Snippet: ( A and B ) Violin plots showing the expression of invadopodium-related genes across cell components in ascites on the basis of two different scRNA-seq datasets from Izar et al. and Zheng et al. . In the dataset of (A), mesothelial cells are classified as fibroblasts. ( C ) Collagen degradation assay. The thickness represents the cell invasion ability. Scale bars, 400 μm. ( D ) Bar graph showing the thickness of remnant collagen 48 hours after incubation. ( E ) Bar graph showing the number of invadopodia. sh-Fascin-1 or sh-myosin X inhibited invadopodium maturation. ( F and G ) 3D images and bar graph showing that spheroids invade collagen with shRNA-induced mesothelial cells (green) and OV90 (red). The invasion ability of mesothelial cells was significantly inhibited by sh- FSCN1 or sh- MYO10 . Scale bars, 200 μm. ( H ) Scheme of the malignant ascites in vivo model using shRNA-treated HPMCs. ( I and J ) Images and bar graph showing the differences in the metastasis area on the omentum from mice 1 week after the injection of OV90 with or without sh-induced mesothelial cells. Scale bars, 1 mm. ( K ) Representative IHC image of mouse tissue with fascin-1. Invasive stromal cells strongly expressed fascin-1. Scale bar, 100 μm. ( L ) IHC of metastasis samples in clinical samples. Fascin-1–positive stromal cells were present in the tumor-invasive regions. Scale bar, 100 μm. ( M ) Kaplan-Meier plot showing the patient’s progression-free survival depending on fascin-1 expression in stromal cells or cancer cells. Fascin-1 expression in stromal cells in metastasis samples was significantly related to a worse prognosis ( P = 0.030). * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: The OV90 (RRID: CVCL_3768), SKOV3 (CVCL_0532), OVCAR3 (CVCL_0465), ES2 (CVCL_3509), and BJ (CRL_2522) cells were obtained from American Type Culture Collection (Manassas, VA).

Techniques: Expressing, Degradation Assay, Incubation, shRNA, In Vivo, Injection

A) Quantitative PCR (qPCR) analysis of Upk3b and Serpinb2 expression in immortalized mesothelial cells (iMC) stimulated with KPCA-conditioned media and Met5a stimulated with patient D HGSOC media (ptD) or Ov90 ovarian cancer cell line in a time-dependent manner (0-96hrs). B) Western blot analysis of UPK3B and SERPINB2 expression in iMC treated with KPCA-condioned culture medium at 0h, 24h, 48h, 72h and 96h (left panel) and Met5A stimulated with conditioned media from ptD/Ov90 cell-lines versus unstimulated controls at 72h (right panel). C) Immunofluorescence analysis of UPK3B and SERPINB2 in iMC and Met5A comparing conditions stimulated with either KPCA-conditioned media or ptD/Ov90 media versus unstimulated controls at 72 h. D) Immunofluorescence staining for ACTA2 and COL4A1 in both primary MCs and immortalized MCs (iMCs) after 96 hours of exposure to KPCA-conditioned media

Journal: bioRxiv

Article Title: Cancer-Associated Mesothelial Cells Drive Immune Escape and Therapy Resistance in Ovarian Cancer

doi: 10.64898/2026.01.07.698232

Figure Lengend Snippet: A) Quantitative PCR (qPCR) analysis of Upk3b and Serpinb2 expression in immortalized mesothelial cells (iMC) stimulated with KPCA-conditioned media and Met5a stimulated with patient D HGSOC media (ptD) or Ov90 ovarian cancer cell line in a time-dependent manner (0-96hrs). B) Western blot analysis of UPK3B and SERPINB2 expression in iMC treated with KPCA-condioned culture medium at 0h, 24h, 48h, 72h and 96h (left panel) and Met5A stimulated with conditioned media from ptD/Ov90 cell-lines versus unstimulated controls at 72h (right panel). C) Immunofluorescence analysis of UPK3B and SERPINB2 in iMC and Met5A comparing conditions stimulated with either KPCA-conditioned media or ptD/Ov90 media versus unstimulated controls at 72 h. D) Immunofluorescence staining for ACTA2 and COL4A1 in both primary MCs and immortalized MCs (iMCs) after 96 hours of exposure to KPCA-conditioned media

Article Snippet: The KPCA cell lines were developed and described in Iyer et al. and the OV90 cells lines of malignant papillary serous adenocarcinoma were purchased from ATCC.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Immunofluorescence, Staining

Review

Structured Review

Images

1) Product Images from "Mesothelial cells promote peritoneal invasion and metastasis of ascites-derived ovarian cancer cells through spheroid formation"

Similar Products

Image Search Results